Fourth Phase Water or exclusion-zone (EZ) water and Rapamycin

General
1

Are there any studies on how rapamycin affects EZ water in the body?

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Hello! Brandon here from the Clinical Team.

To provide a quick outline for the audience: Exclusion Zone (EZ) water is still a theoretical, controversial field. Imagine when water touches certain surfaces inside your body (like cell membranes or proteins), the water molecules line up in a neat, organized way instead of being jumbled. This neat layer of water pushes away particles and waste, creating a “clean zone” (the exclusion zone). The structured water layer is slightly negatively charged, and the nearby regular water is more positive. That separation of charges is like a tiny battery inside your body. Light (especially infrared, like warmth from the sun or body heat) can make this “battery” layer bigger.

Some proposed benefits are:

  • More energy: The charge separation could give cells an extra energy boost, like topping up a battery.

  • Cell cleanup: By pushing away junk and waste, it may help cells stay organized (some overlap here with the autophagy process of approaches such as low dose rapamycin, intermittent fasting, etc.)

  • Hydration: EZ water may improve how water is stored and used in tissues.

On the other hand, the unknowns include:

  • Not proven yet: Most of this research is experimental in the lab, not fully confirmed in living humans.

  • Overhyped products: Some supplements or “structured water” drinks make big claims without real evidence.

  • No direct harms known, but the main “risk” is relying on it instead of proven health approaches.

I took a look, and there aren’t peer-reviewed studies directly testing rapamycin’s effects on EZ water (exclusion-zone/fourth-phase water) in either humans or animals. I couldn’t find any paper that measures EZ size or properties before/after rapamycin exposure.

So, we must rely on what we do know and offer hypotheses based on this:

  • The EZ water concept has some experimental reports but is still controversial, with active debate about mechanisms and biological significance.

  • There are lab studies showing certain health-promoting agents can change EZ size in model systems (e.g., around Nafion), but rapamycin hasn’t been tested in those paradigms.

  • Independently, rapamycin clearly modulates mitochondrial function and mTOR signaling (e.g., alters respiration, oxidative capacity, autophagy), but none of these mainstream studies assess EZ water.

Bottom line: there’s no direct evidence tying rapamycin to changes in EZ/“structured” water. If you’re exploring rapamycin mechanisms, the strongest literature remains on mTORC1 inhibition → autophagy/mitophagy, shifts in mitochondrial respiration, and downstream metabolic effects—not EZ water.