# Fourth Phase Water or exclusion-zone (EZ) water and Rapamycin

**URL:** <https://community.gethealthspan.com/t/fourth-phase-water-or-exclusion-zone-ez-water-and-rapamycin/2005>\
**Category:** General\
**Created:** [September 20, 2025, 2:41am UTC](https://community.gethealthspan.com/t/fourth-phase-water-or-exclusion-zone-ez-water-and-rapamycin/2005 "2025-09-20T02:41:13Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![charter](https://avatars.discourse-cdn.com/v4/letter/c/f475e1/32.png) [@charter](https://community.gethealthspan.com/u/charter)\
**Post date:** [September 20, 2025, 2:41am UTC](https://community.gethealthspan.com/t/fourth-phase-water-or-exclusion-zone-ez-water-and-rapamycin/2005/1 "2025-09-20T02:41:13Z")

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Are there any studies on how rapamycin affects EZ water in the body?

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**Author:** ![brandon](https://avatars.discourse-cdn.com/v4/letter/b/f08c70/32.png) [@brandon](https://community.gethealthspan.com/u/brandon)\
**Post date:** [September 20, 2025, 12:19pm UTC](https://community.gethealthspan.com/t/fourth-phase-water-or-exclusion-zone-ez-water-and-rapamycin/2005/2 "2025-09-20T12:19:58Z")

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Hello! Brandon here from the Clinical Team.

To provide a quick outline for the audience: Exclusion Zone (EZ) water is still a theoretical, controversial field. Imagine when water touches certain surfaces inside your body (like cell membranes or proteins), the water molecules line up in a neat, organized way instead of being jumbled. This neat layer of water pushes away particles and waste, creating a “clean zone” (the exclusion zone). The structured water layer is slightly negatively charged, and the nearby regular water is more positive. That separation of charges is like a tiny battery inside your body. Light (especially infrared, like warmth from the sun or body heat) can make this “battery” layer bigger.

Some proposed benefits are:

- **More energy:** The charge separation could give cells an extra energy boost, like topping up a battery.

- **Cell cleanup:** By pushing away junk and waste, it may help cells stay organized (some overlap here with the autophagy process of approaches such as low dose rapamycin, intermittent fasting, etc.)

- **Hydration:** EZ water may improve how water is stored and used in tissues.

On the other hand, the unknowns include:

- **Not proven yet:** Most of this research is experimental in the lab, not fully confirmed in living humans.

- **Overhyped products:** Some supplements or “structured water” drinks make big claims without real evidence.

- **No direct harms known** , but the main “risk” is relying on it instead of proven health approaches.

I took a look, and there aren’t peer-reviewed studies directly testing rapamycin’s effects on EZ water (exclusion-zone/fourth-phase water) in either humans or animals. I couldn’t find any paper that measures EZ size or properties before/after rapamycin exposure.

So, we must rely on what we _do_ know and offer hypotheses based on this:

- The EZ water concept has some experimental reports but is still controversial, with active debate about mechanisms and biological significance.

- There _are_ lab studies showing certain health-promoting agents can change EZ size in model systems (e.g., around Nafion), but rapamycin hasn’t been tested in those paradigms.

- Independently, rapamycin clearly modulates mitochondrial function and mTOR signaling (e.g., alters respiration, oxidative capacity, autophagy), but none of these mainstream studies assess EZ water.

Bottom line: there’s no direct evidence tying rapamycin to changes in EZ/“structured” water. If you’re exploring rapamycin mechanisms, the strongest literature remains on mTORC1 inhibition → autophagy/mitophagy, shifts in mitochondrial respiration, and downstream metabolic effects—not EZ water.